Technical Reference #523

523.

Texture Analysis Of Fluorescence Lifetime Images Of Nuclear Dna With Effect Of Fluorescenceresonance Energy Transfer Shin-ichi Murata; Petr Herman; and Joseph R. Lakowicz, University of Maryland, Cytometry, 43(523), (2001)
Link To Paper

Abstract:
Fluorescence lifetime imaging microscopy(FLIM) is becoming an important tool in cellular imaging.In FLIM the image contrast is concentration insensitivewhereas it is sensitive to the local environment and interactionsof fluorophores such as fluo

Keywords:
3T3 fibroblasts; Hoechst 33258; 7-aminoactinomycin D; cell cycle

Materials & Methods:
Mouse fibroblasts (3T3-Swiss albino) were grown at 37°C in the bottom-glass dishes (Mat Tek Ashland MA) containing Dulbecco’s modified Eagles’s medium with 10% calf serum.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
3T3