Technical Reference #521
Glass Bottom Culture Dishes
This study used MatTek product(s):
Citation in paper containing MatTek reference:
35-mm glass-bottomed microwell dish (Mat Tek) 
521. |
Catalytic Activity Is Required For Calcium/Calmodulin-Dependent Protein Kinase Iv To Enter The Nucleus
Shannon M. Lemrow; Kristin A. Anderson; James D. Joseph; Thomas J. Ribar;
Pamela K. Noeldner; and Anthony R. Means,
Duke University,
The Journal of Biological Chemistry,
279(521),
(2004)
Link To Paper
Abstract:
Calcium/calmodulin-dependent protein kinase IV(CaMKIV) is a nuclear protein kinase that responds toacute rises in intracellular calcium by phosphorylatingand activating proteins involved in transcription. Consistentwith these roles CaMKIV is foun Keywords:
Catalytic Activity; Protein Kinase IV; calcium; nucleus; activation loop phosphorylation Materials & Methods:
HEK293A cells were plated at a density of 0.1 106/35-mm glass-bottomed microwell dish (Mat Tek).
The DNA:Opti-MEM ratio was 0.5 g of the indicated constructs (see
figure legends) into 50 l of Opti-MEM (Invitrogen) and the LipofectAMINE:
Opti-MEM r Microscopic Technique
Confocal Microscopy Cell Type(s)
HEK-293 |