Technical Reference #521

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm glass-bottomed microwell dish (Mat Tek)

521.

Catalytic Activity Is Required For Calcium/Calmodulin-Dependent Protein Kinase Iv To Enter The Nucleus Shannon M. Lemrow, Kristin A. Anderson, James D. Joseph, Thomas J. Ribar, Pamela K. Noeldner, and Anthony R. Means, Duke University, The Journal of Biological Chemistry, 279(12), 11665, (2004)
Link To Paper

Abstract:
Calcium/calmodulin-dependent protein kinase IV(CaMKIV) is a nuclear protein kinase that responds toacute rises in intracellular calcium by phosphorylatingand activating proteins involved in transcription. Consistentwith these roles, CaMKIV is foun

Keywords:
Catalytic Activity, Protein Kinase IV, calcium, nucleus, activation loop phosphorylation

Materials & Methods:
HEK293A cells were plated at a density of 0.1 106/35-mm glass-bottomed microwell dish (Mat Tek). The DNA:Opti-MEM ratio was 0.5 g of the indicated constructs (see figure legends) into 50 l of Opti-MEM (Invitrogen), and the LipofectAMINE: Opti-MEM r

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HEK-293