Technical Reference #521

521.

Catalytic Activity Is Required For Calcium/Calmodulin-Dependent Protein Kinase Iv To Enter The Nucleus Shannon M. Lemrow; Kristin A. Anderson; James D. Joseph; Thomas J. Ribar; Pamela K. Noeldner; and Anthony R. Means, Duke University, The Journal of Biological Chemistry, 279(521), (2004)
Link To Paper

Abstract:
Calcium/calmodulin-dependent protein kinase IV(CaMKIV) is a nuclear protein kinase that responds toacute rises in intracellular calcium by phosphorylatingand activating proteins involved in transcription. Consistentwith these roles CaMKIV is foun

Keywords:
Catalytic Activity; Protein Kinase IV; calcium; nucleus; activation loop phosphorylation

Materials & Methods:
HEK293A cells were plated at a density of 0.1 106/35-mm glass-bottomed microwell dish (Mat Tek). The DNA:Opti-MEM ratio was 0.5 g of the indicated constructs (see figure legends) into 50 l of Opti-MEM (Invitrogen) and the LipofectAMINE: Opti-MEM r

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HEK-293