Technical Reference #520

520.

Mitochondrial Survivin Inhibits Apoptosis And Promotes Tumorigenesis Takehiko Dohi; Elena Beltrami; Nathan R. Wall; Janet Plescia; and Dario C. Altieri, UMASS Medical Center, The Journal of Clinical Investigation, 114(520), (2004)
Link To Paper

Abstract:
Evasion of apoptosis is a hallmark of cancer but the molecular circuitries of this process are not understood.Here we show that survivin a member of the inhibitor of apoptosis gene family that is overexpressed in cancerexists in a novel mitochondri

Keywords:
apoptosis; tumorigenesis; cancer; mitochondrial survivin

Materials & Methods:
INS-1 stable transfectants were plated onto 35-mm glass-bottom culture dishes with grid (MatTek Corp.). After 24 hours cells were treated with staurosporine (Sigma-Aldrich) washed with PBS and scanned with a manual/motorized reflected-fluorescence s

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
HeLa