Technical Reference #518

518.

Zn2+ Currents Are Mediated By Calcium-Permeable Ampa/Kainate Channels In Cultured Murine Hippocampal Neurones Yousheng Jia; Jade-Ming Jeng; Stefano L. Sensi; John H. Weiss, University of California Irivine, The Journal of Physiology, 543(518), (2002)
Link To Paper

Abstract:
Permeation of the endogenous cation Zn2+ through calcium-permeable AMPA/kainate receptorgated(Ca-A/K) channels might subserve pathological and/or physiological signalling roles.Voltage-clamp recording was used to directly assess Zn2+ flux through thes

Keywords:
endogenous cation Zn2+; Voltage-clamp recording; AMPA/kainate receptorgated (Ca-A/K) channels; rectification

Materials & Methods:
Mixed hippocampal cell cultures were prepared from fetal mice gestational age 15–16 days generally as previously described (Yin et al. 1999). Pregnant Swiss-Webster mice were deeply anaesthetized using 4 % halothane killed by cervical dislocation a

Microscopic Technique
Inverted Microscopy

Cell Type(s)
Neurons - Hippocampal