Technical Reference #515

515.

Importance Of Nuclear Localization Of Apoptin For Tumor-Specificinduction Of Apoptosis Astrid A. A. M. Danen-van Oorschot, Ying-Hui Zhang, S. Rutger Leliveld, Jennifer L. Rohn, Maud C. M. J. Seelen, Marian W. Bolk, Arend van Zon, Stefan J. Erkeland, Jan-Pieter Abrahams, Dominik Mumberg, and Mathieu H. M. Noteborn, Leiden University, The Journal of Biological Chemistry, 278(30), 27730, (2003)
Link To Paper

Abstract:
The chicken anemia virus-derived protein Apoptin induces apoptosis specifically in human tumor and transformedcells and not in normal, untransformed cells.The cell killing activity correlates with a predominantlynuclear localization of Apoptin in t

Keywords:
Apoptin, Apoptosis, nuclear localization, chicken anemia virus, cancer

Materials & Methods:
For microinjections Saos-2 cells were cultured on glass-bottomed microinjection dishes (MatTek Corporation) or in 35-mm dishes containing glass Cellocate coverslips (Eppendorf). The cells were microinjected in the nucleus with DNA (50 ng/l in phosphat

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Saos-2