Technical Reference #515

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass-bottomed microinjection dishes (MatTek Corporation)

515.

Importance Of Nuclear Localization Of Apoptin For Tumor-Specificinduction Of Apoptosis Astrid A. A. M. Danen-van Oorschot; Ying-Hui Zhang; S. Rutger Leliveld; Jennifer L. Rohn; Maud C. M. J. Seelen; Marian W. Bolk; Arend van Zon; Stefan J. Erkeland; Jan-Pieter Abrahams; Dominik Mumberg; and Mathieu H. M. Noteborn, Leiden University, The Journal of Biological Chemistry, 278(515), (2003)
Link To Paper

Abstract:
The chicken anemia virus-derived protein Apoptin induces apoptosis specifically in human tumor and transformedcells and not in normal untransformed cells.The cell killing activity correlates with a predominantlynuclear localization of Apoptin in t

Keywords:
Apoptin; Apoptosis; nuclear localization; chicken anemia virus; cancer

Materials & Methods:
For microinjections Saos-2 cells were cultured on glass-bottomed microinjection dishes (MatTek Corporation) or in 35-mm dishes containing glass Cellocate coverslips (Eppendorf). The cells were microinjected in the nucleus with DNA (50 ng/l in phosphat

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Saos-2