Technical Reference #512

512.

Group Ivc Cytosolic Phospholipase A2Is Farnesylated And Palmitoylated In Mammalian Cells Dawn E. Tucker; Allison Stewart; Laxman Nallan; Pravine Bendale; Farideh Ghomashchi; Michael H. Gelb; and Christina C. Leslie, National Jewish Medical and Research Center, Journal of Lipid Research, 46(512), (2005)
Link To Paper

Abstract:
Cytosolic phospholipase A2 (cPLA 2 is a member of the group IV family of intracellular phospholipase A 2 enzymes but unlike the well-studied cPLA2 it is constitutivelybound to membrane and is calcium independent.cPLA2 co

Keywords:
prenylation; fatty acylation; mitochondria

Materials & Methods:
IMLF / or SkMc cells were plated on 35 mm glass-bottomed MatTek plates at 1.25 104 cells/cm2 and infected with AdcPLA2 as described above. The cells were rinsed once with PBS and incubated for 15 min in ice-cold fixative containing 3.2% paraforma

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
SkMc cells