Technical Reference #511

511.

Directed Delivery Of Heat-Labile Enterotoxin By Enterotoxigenicescherichia Coli F. Chuck Dorsey; Julia F. Fischer and James M. Fleckenstein, University of Tennessee, Cell Microbiology, 8(511), (2006)
Link To Paper

Abstract:
EnterotoxigenicEscherichia coli(ETEC) leading causes of diarrhoeal morbidity and mortality in developingcountries are heterogenous pathogens thatelaborate heat-labile (LT) and/or heat-stable (ST)enterotoxins which elicit watery cholera-like

Keywords:
heat-labile enterotoxin; enterotoxigenic Escherichia coli; host–pathogen interaction; type II secretion system

Materials & Methods:
To obtain real-time images of bacteria expressing GFP-labelled proteins interacting with host cells either HCT-8 or CaCo-2 cells were grown overnight at low density on glass bottom culture dishes (MatTek Ashland MA). Strain jf1128 was grown overnigh

Microscopic Technique
Electron Microscopy, Scanning

Cell Type(s)
HCT-8