Technical Reference #508

508.

Superoxide Production At Phagosomal Cup/Phagosome Through I Protein Kinase C During Fc R-Mediated Phagocytosis In Microglia Takehiko Ueyama; Michelle R. Lennartz; Yukiko Noda; Toshihiro Kobayashi; Yasuhito Shirai; Kyoko Rikitake; Tomoko Yamasaki; Shigeto Hayashi; Norio Sakai; Harumichi Seguchi; Makoto Sawada; Hideki Sumimoto; and Naoaki Saito, Kobe University, The The Journal of Immunology, 173(508), (2004)
Link To Paper

Abstract:
Protein kinase C (PKC) plays a prominent role in immune signaling. To elucidate the signal transduction in a respiratory burstand isoform-specific function of PKC during Fc R-mediated phagocytosis we used live digital fluorescence imaging of mousemi

Keywords:
Superoxide Production; Phagocytosis; Protein kinase C; O2- production; Alzheimer’s disease

Materials & Methods:
The 6-3 microglial cells are generated by spontaneous immortalization of primary microglia from op/op mice which are deficient of M-CSF-derived macrophages and severely monocytopenic (15 16). The 6-3 cells closely resemble primary microglia with respe

Microscopic Technique
Confocal Microscopy

Cell Type(s)
6-3