Technical Reference #507

507.

In Vivo Selective Cytoskeleton Dynamics Quantification In Interphase Cells Induced By Pulsed Ultraviolet Laser Nanosurgery Julien Colombellia; Emmanuel G. Reynauda; Jens Rietdorf; Rainer Pepperkok and Ernst H.K. Stelzer, European Molecular Biology Laboratory, Traffic, 6(507), (2005)
Link To Paper

Abstract:
We report on the manipulation of intracellular filaments using a nanosurgery system based on a subnanosecondpulsed UV laser optimized for the localized severing ofbiological polymers. By inducing artificial catastrophe ofselected microtubules (MTs)

Keywords:
stress fibers; dynamic instability; microtubule; nanosurgery; pulsed laser

Materials & Methods:
Wild-type Ptk2 cells were grown in DMEM supplemented with 10% fetal bovine serum under standard tissue culture conditions. All cell-culture media and sera were obtained from Invitrogen (Carlsbad CA USA). Ptk2 cells stably expressing tagged tubulin-YF

Microscopic Technique
Fluorescence Microscopy, Total Internal Reflection Fluorescence (TIRF), Laser nanosurgery microscopy

Cell Type(s)
Ptk2