Technical Reference #506

506.

Increases In Estrogen Receptor-Concentration In Breast Cancer Cells Promote Serine 118/104/106- Independent Af-1 Transactivation And Growth In The Absence Of Estrogen AMY M. FOWLER; NATALIA SOLODIN; MARA T. PREISLER-MASHEK; PING ZHANG; ADRIAN V. LEE; AND ELAINE T. ALARID, University of Wisconsin-Madison, FASEB, 18(506), (2004)
Link To Paper

Abstract:
A common phenotype in breast cancer isthe expansion of the estrogen receptor- (ER ) cellpopulation and an inappropriate elevation of ER protein the latter predisposing patients for a poorerprognosis than those with lower levels of the receptor.

Keywords:
nuclear receptor; steroid hormone; transcription; proliferation; ligand-independent

Materials & Methods:
ERHA cells were grown on confocal-ready tissue culture plates (MatTek Corporation Ashland MA USA) and treated with vehicle or 1 g/mL Dox for 48 h. Cells were subsequently fixed overnight with 3.7% formaldehyde in PBS at 4°C. A solution of 3.7% fo

Microscopic Technique
Confocal Microscopy, Laser Scanning

Cell Type(s)
ERHA