Technical Reference #505

505.

Planktonic Replication Is Essential For Biofilm Formation By Legionella Pneumophila In A Complex Medium Under Static And Dynamic Flow Conditions Jo¨rg Mampel; Thomas Spirig; Stefan S. Weber; Janus A. J. Haagensen; Søren Molin; and Hubert Hilbi, Swiss Federal Institute of Technology, Applied and Environmental Microbiology, 72(505), (2006)
Link To Paper

Abstract:
Legionella pneumophila persists for a long time in aquatic habitats where the bacteria associate with biofilmsand replicate within protozoan predators. While L. pneumophila serves as a paradigm for intracellular growthwithin protozoa it is less clea

Keywords:
Planktonic Replication; Legionella pneumophila; protozoa; axenic replication; planktonic bacteria

Materials & Methods:
The architecture of GFP- and DsRedlabeled L. pneumophila biofilms was analyzed in glass-bottom microwell dishes (35-mm petri dishes; MatTek) under static conditions (no medium replacement) or quasi-static conditions (medium replaced twice a day). An in

Microscopic Technique
Confocal Microscopy

Cell Type(s)
L