Technical Reference #502

502.

Hmg-Coa Reductase Inhibitors Suppress Intracellular Calcium Mobilization And Membrane Current Induced By Lysophosphatidylcholine In Endothelial Cells Keiko Yokoyama; MD; Toshiyuki Ishibashi; MD; Hiroshi Ohkawara; MD; Junko Kimura; MD; Isao Matsuoka; PhD; Takayuki Sakamoto; MD; Kenji Nagata; MD; Koichi Sugimoto; MD; Sotaro Sakurada; MD; Yukio Maruyama; MD, Fukushima Medical University, Circulation, 105(502), (2002)
Link To Paper

Abstract:
Lysophosphatidylcholine (LPC) is known to increase intracellular Ca2 concentration ([Ca2 ]i) in endothelialcells. This study was conducted to investigate the effects of HMG-CoA reductase inhibitors (statins) on the increasein [Ca2 ]I and membrane cur

Keywords:
endothelium; signal transduction; statins; calcium; ion channels

Materials & Methods:
[Ca2]i was measured as described previously.10 Briefly endothelial cells were grown to confluence in 35-mm glass-bottom dishes (MatTek). The cells were incubated at 37°C for 10 minutes with 1 mol/L fura-2/acetoxymethyl ester and then washed with the

Microscopic Technique
Fluorescence Microscopy, Inverted Fluorescence Microscopy

Cell Type(s)
Endothelial