Technical Reference #501

501.

Use Of Confocal Microscopy To Analyze The Rate Of Vancomycin Penetration Through Staphylococcus Aureus Biofilms Kimberly K. Jefferson; Donald A. Goldmann; and Gerald B. Pier, Brigham & Women's Hospital, Antimicrobial Agents and Chemotherapy, 49(501), (2005)
Link To Paper

Abstract:
When bacteria assume the biofilm mode of growth they can tolerate levels of antimicrobial agents 10 to 1000times higher than the MICs of genetically equivalent planktonic bacteria. The properties of biofilms that give riseto antibiotic resistance ar

Keywords:
biofilm; Vancomycin Penetration; Staphylococcus aureus; bacteria

Materials & Methods:
Biofilms were grown overnight in TSBG in 35-mm collagen-coated glass coverslip-bottom dishes (MatTek Co. Ashland MA). The following day the media were removed and the biofilm was washed once in H2O. All confocal images were obtained at the Harvard

Microscopic Technique
Confocal Microscopy

Cell Type(s)
S. aureus