Technical Reference #499

499.

Production Of Cell Lines Secreting Tat Fusion Proteins Tibor Barka; Edward S. Gresik; and Scott C. Henderson, Mount Sinai School of Medicine, The Journal of Histochemistry & Cytochemistry, 52(499), (2004)
Link To Paper

Abstract:
Transduction of proteins and other macromolecules constitutes a potent technology to analyze cell functions and to achieve therapeutic interventions. In generalfusion proteins with protein transduction domains such as TAT are produced in a bacterial

Keywords:
TAT fusion proteins; green fluorescent protein; red fluorescent protein; mammalian expression vector

Materials & Methods:
We examined most cultures without fixation with a confocal microscope [Leica TCS-SP (UV)] or a Zeiss Axiphot fluorescence microscope. For observations of living cells they were grown in Glass Bottom Microwell Dishes (MatTek; Ashland MA). However so

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
CHO