Technical Reference #496

496.

Activation Of Erbb-1 Signaling In Tanycytes Of The Median Eminence Stimulates Transforming Growth Factor 1Release Via Prostaglandin E2 Production And Induces Cellplasticity Vincent Prevot, Anda Cornea, Alison Mungenast, Gregory Smiley, and Sergio R. Ojeda, Oregon Health and Sciences University, The The Journal of Neuroscience, 23(33), 10624, (2003)
Link To Paper

Abstract:
The activation of transforming growth factor (TGF)– erbB-1 and neuregulin– erbB-4 signaling pathways in hypothalamic astrocyteshas been shown to play a key role in the process by which the neuroendocrine brain controls luteinizing hormone-releasing ho

Keywords:
neuroendocrine; TGF ; TGF; ependymoglial cells; LHRH; cell plasticity; hypothalamus

Materials & Methods:
Tanycytes were seeded in cloning cylinders positioned on 37 mm culture chambers (MatTek Corp., Ashland, MA) containing poly-L-ornithine- and laminin-coated glass coverslips, as described above. After removal of the cylinder, the cells were maintained

Microscopic Technique
Fluorescence Microscopy, Axioscope

Cell Type(s)
Tanycytes