Technical Reference #494

494.

Large-Scale Chromatin Decondensation And Recondensation Regulated By Transcription From A Natural Promoter Waltraud G. Müller; Dawn Walker; Gordon L. Hager; and James G. McNally, National Institutes of Health, The Journal of Cell Biology, 154(494), (2001)
Link To Paper

Abstract:
We have examined the relationship between transcription and chromatin structure using a tandemarray of the mouse mammary tumor virus(MMTV) promoter driving arasreporter. The array was visualizedas a distinctive fluorescent structure in live cel

Keywords:
chromatin decondensation and recondensation; transcription; mouse mammary tumor virus (MMTV); dipteran flies; Chromosome puffs; lampbrush chromosomes

Materials & Methods:
To provide assurance that our observations were not affected by imaging conditions we used three different methods for time-lapse analysis of MMTV arrays. First cells were grown in a Petri dish with a gridded glass bottom (MatTek Corporation). Partic

Microscopic Technique
Deconvolution Microscopy

Cell Type(s)
3617