Technical Reference #492

492.

Atomic Force-Multi-Optical Imaging Integrated Microscope For Monitoring Molecular Dynamics In Live Cells Andreea Trache, Gerald A. Meininger, Texas A&M University, Journal of Biomedical Optics, 10, 064023-10, (2005)
Link To Paper

Abstract:
A novel hybrid imaging system is constructed integrating atomic force microscopy AFM with a combination of optical imaging techniques that offer high spatial resolution. The main applicationof this instrument the NanoFluor microscope is the study

Keywords:
integrated microscopy; atomic force microscopy; total internal reflection fluorescence; Forster resonance energy transfer; internal reflection microscopy

Materials & Methods:
The VSMCs were isolated from a rat cremaster arteriole as previously described.68 Low passage VSMCs were trypsinized and then centrifuged to form a pellet. The cell pellet was dispersed in cell culture media, and the cells were cultured on glass-botto

Microscopic Technique
Scanning Probe Microscopy, Atomic Force Microscopy (AFM)

Cell Type(s)
VSMCs