Technical Reference #491

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35 mm glass-bottom poly-l-lysine-coated culture dishes were purchased from MatTek Corp. (Ashland, MA)

491.

Ceramide Inhibition Of Nf-Kb Activation Involves Reverse Translocation Of Classical Protein Kinase C (Pkc) Isoenzymes: Requirement For Kinase Activityand Carboxyl-Terminal Phosphorylation Of Pkc For The Ceramide Response PAOLA SIGNORELLI, CHIARA LUBERTO, AND YUSUF A. HANNUN, Medical University of South Carolina, FASEB, 15, 2402, (2001)
Link To Paper

Abstract:
Protein kinase C (PKC) is known toactivate NF-kB whereas the lipid mediator ceramidewas recently shown to inhibit activation of this transcriptionfactor (1, 2). In this study, the mechanisms bywhich ceramide interferes with this pathway were exami

Keywords:
diacylglycerol, sphingolipid, glycerolipid, C6-ceramide

Materials & Methods:
Jurkat T cells and MCF7 adenocarcinoma cells were obtained from ATCC (Rockville, MD). TNF-a was purchased from PeproTech (Rocky Hill, NJ); C6-ceramide was purchased from Matreya (Pleasant Gap, PA); [g-32]ATP was purchased from NEN Life Science Product

Microscopic Technique
Confocal Microscopy

Cell Type(s)
MCF-7