Technical Reference #490

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
polylysine-coated glass-bottomed 35 mmdiameter filming dishes (MatTek Corporation, Ashland, MA)

490.

Behaviour Of Drg Sensory Neurites At The Intact And Injured Adult Ratdorsal Root Entry Zone: Postnatal Neurites Become Paralysed,Whilst Injury Improves The Growth Of Embryonic Neurites JON P. GOLDING, CHARLES BIRD, STEPHEN MCMAHON, AND JAMES COHEN, King's College London, GLIA, 26, 311, (1999)
Link To Paper

Abstract:
The dorsal root entry zone is a PNS-CNS junction between Schwanncells and astrocytes, defining the site where dorsal root ganglia (DRG) axons enter theadult mammalian spinal cord. Following dorsal root injury (rhizotomy), DRG axonsregenerate within

Keywords:
neurite growth inhibitor; PNS; CNS; glial interface; time-lapse

Materials & Methods:
A total of 12 adult Wistar rats were anaesthetised with pentobarbitone (40 mg/kg ip). The cauda equina was exposed by dorsal laminectomy and the dorsal roots (DR) S1, S2, and L1 were completely transected on the right side of the body approximately 1

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Schwann