Technical Reference #490

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
polylysine-coated glass-bottomed 35 mmdiameter filming dishes (MatTek Corporation; Ashland; MA)

490.

Behaviour Of Drg Sensory Neurites At The Intact And Injured Adult Ratdorsal Root Entry Zone: Postnatal Neurites Become Paralysed;Whilst Injury Improves The Growth Of Embryonic Neurites JON P. GOLDING; CHARLES BIRD; STEPHEN MCMAHON; AND JAMES COHEN, King's College London, GLIA, 26(490), (1999)
Link To Paper

Abstract:
The dorsal root entry zone is a PNS-CNS junction between Schwanncells and astrocytes defining the site where dorsal root ganglia (DRG) axons enter theadult mammalian spinal cord. Following dorsal root injury (rhizotomy) DRG axonsregenerate within

Keywords:
neurite growth inhibitor; PNS; CNS; glial interface; time-lapse

Materials & Methods:
A total of 12 adult Wistar rats were anaesthetised with pentobarbitone (40 mg/kg ip). The cauda equina was exposed by dorsal laminectomy and the dorsal roots (DR) S1 S2 and L1 were completely transected on the right side of the body approximately 1

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Schwann