Technical Reference #488

488.

Impaired Glucagon Secretory Responses In Mice Lacking The Type 1 Sulfonylurea Receptor Chiyo Shiota; Jonathan V. Rocheleau; Masakazu Shiota; David W. Piston; and Mark A. Magnuson, Vanderbilt University, American Journal of Physiology: Endocrinology and Metabolism, 289(488), (2005)
Link To Paper

Abstract:
Impaired glucagonsecretory responses in mice lacking the type 1 sulfonylurea receptor.Am J Physiol Endocrinol Metab 289: E570–E577 2005. First publishedJune 7 2005; doi:10.1152/ajpendo.00102.2005.—Pancreatic -cells like -cells express ATP-sen

Keywords:
pancreatic -cell; adenosine 5'-triphosphate-sensitive potassium channel; sulfonylurea receptor 1; pancreas perfusion; glucagon

Materials & Methods:
Single islet cells were suspended in RPMI 1640 medium (GIBCO/ Invitrogen) supplemented with 10% FBS 11 mM glucose 100 IU/ml penicillin and 100 g/ml streptomycin and allowed to attach to poly-L-lysine-coated gridded glass-bottom culture dishes (MatT

Microscopic Technique
Inverted Microscopy, Inverted Phase Contrast Photo microscopy

Cell Type(s)
Islet