Technical Reference #487

487.

Determining Protease Activity In Vivo By Fluorescence Cross-Correlation Analysis Tobias Kohl; Elke Haustein; and Petra Schwille, Max-Planck Institute, Biophysical Journal, 89(487), (2005)
Link To Paper

Abstract:
To date most biochemical approaches to unravel protein function have focused on purified proteins in vitro.Whereas they analyze enzyme performance under assay conditions they do not necessarily tell us what is relevant withina living cell. Ideally

Keywords:
Protease Activity; protein; Fluctuation-based fluorescence techniques

Materials & Methods:
ER293 cells were obtained from Stratagene (Heidelberg Germany) and grown in an 8.5% CO2-humidified atmosphere at 37 C in 88% (v/v) phenolred free Dulbecco’s modified Eagle’s medium (GibcoBRL Karlsruhe Germany) containing 10% (v/v) Mycoplex Fetal Cal

Microscopic Technique
Inverted Microscopy

Cell Type(s)
ER293