Technical Reference #486

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass coverslip at the bottom of a microwell dish (35-mm dish; poly-D-lysine-coated; MatTek; Ashland; MA; USA)

486.

Mitochondrial Transmembrane Potential Is Diminished In Phorbol Myristate Acetatestimulated Peritoneal Resident Macrophagesisolated From Wild-Type Mice; But Not In Thosefrom Gp91-Phox-Deficient Mice Toshihiro Kobayashi; Yasuhiro Ogawa; Yoshiya Watanabe; Masato Furuya; Sayo Kataoka; Eva Garcia del Saz; Shohko Tsunawaki; Mary C. Dinauer and Harumichi Seguchi, Kochi University, Histochemistry and Cell Biology, 122(486), (2004)
Link To Paper

Abstract:
Macrophages produce superoxide (O2–) during phagocytosis or upon stimulation witha variety of agents including phorbol myristate acetate (PMA) through the activation of NADPHoxidase and the formed O2– is converted to other reactive oxygen species

Keywords:
Apoptosis; Macrophages; Mitochondria; NADPH oxidase; Superoxide

Materials & Methods:
Resident macrophages were isolated from peritoneal cavities of wild-type mice (C57BL/6) and gp91-phox-deficient knockout mice by the method of Badwey et al. (1983). Briefly mice were killed by cervical dislocation under anesthesia with diethyl ether a

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
C57BL/6