Technical Reference #484

484.

Astrocytes Regulate Developmental Changes In The Chloride Ion Gradient Of Embryonic Rat Ventral Spinal Cord Neurons In Culture Yong-Xin Li, Anne E. Schaffner, Marc K. Walton and Jeffery L. Barker, National Institutes of Health, The Journal of Physiology, 509, 848, (1998)
Link To Paper

Abstract:
Embryonic rat ventral spinal cord neurons were dissociated at day 15 and grown on: (i) polyd-lysine (PDL); (ii) a confluent monolayer of type I astrocytes; or (iii) PDL in astrocyteconditionedmedium (ACM) to examine the influence of astroglia on the r

Keywords:
chloride ion gradient, GABAergic potential, amino acids, glial fibrillary acid protein (GFAP),

Materials & Methods:
Pregnant Sprague—Dawley rats were narcotized by COµ inhalation followed by cervical dislocation. E15 embryos were removed from the uteri by Caesarean section, rapidly decapitated and placed in phosphate-buffered saline at room temperature (20—22 °C).

Microscopic Technique
Video Microscopy, Digital

Cell Type(s)
E15