Technical Reference #483

483.

Surface Probe Measurements Of The Elasticity Of Sectioned Tissue; Thin Gels And Polyelectrolyte Multilayer Films:Correlations Between Substrate Stiffness And Cell Adhesion Adam J. Engler; Ludovic Richert; Joyce Y. Wong; Catherine Picart; Dennis E. Discher, University of Pennsylvania, Surface Science, 570(483), (2004)

Abstract:
Surface probe measurements of the elasticity of thin film matrices as well as biological samples prove generallyimportant to understanding cell attachment across such systems. To illustrate this sectioned arteries were probed byatomic force microscop

Keywords:
Surface structure; morphology; roughness; and topography; Adhesion; Atomic force microscopy

Materials & Methods:
A7r5 SMCs (aorta-derived cell line) were maintained in polystyrene flasks between passages 2 and 15 and cultured in media containing DMEM supplemented with 10% of fetal bovine serum and antibiotics. Cells were passed every 3 days when reaching 80

Microscopic Technique
Scanning Probe Microscopy, Atomic Force Microscopy (AFM)

Cell Type(s)
A7r5