Technical Reference #481

481.

Development And Characterization Of An In Vivo Central Venous Catheter Candida Albicans Biofilm Model D. Andes; J. Nett; P. Oschel; R. Albrecht; K. Marchillo; and A. Pitula, University of Wisconsin-Madison, Infection and Immunity, 72(481), (2004)
Link To Paper

Abstract:
Biofilms represent a niche for microorganisms where they are protected from both the host immune systemand antimicrobial therapies. Biofilm growth serves as an increasing source of clinical infections. Candidainfections are difficult to manage due to

Keywords:
Candida albicans; rat central venous catheter model; biofilm matrix

Materials & Methods:
Stained catheter segments were transferred to a glass-bottom petri dish (coverslip 1.5 35-mm disk P325G 1.5-14C; MatTek Ashland Mass.) in an on-end orientation. Biofilms were observed with a Nipkow disk-based confocal microscope (Axiovert 200; Zeiss

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Catheter segments