Technical Reference #480

480.

Heterotrimeric G Proteins Precouple With G Protein-Coupled Receptors In Living Cells Muriel Nobles; Amy Benians; and Andrew Tinker, British Heart Foundation Laboratories, PNAS, 102(480), (2005)
Link To Paper

Abstract:
Using fluorescence resonance energy transfer (FRET) microscopy we investigate how heterotrimeric G proteins interact with Gprotein-coupled receptors (GPCRs). In the absence of receptoractivation the 2A adrenergic and muscarinic M4 receptors arep

Keywords:
heterotrimeric G proteins; G protein-coupled receptors (GPCRs); G protein-gated K channel

Materials & Methods:
Cells for imaging were subcultured onto 25-mm glass coverslips and then placed into a watertight cell imaging chamber at room temperature or were subcultured onto 35-mm culture dishes with integral no. 0 glass coverslip bottoms (Mattek).

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HEK-293