Technical Reference #479

479.

Sindbis Virus-Induced Neuronal Death Is Both Necrotic And Apoptotic And Is Ameliorated By N-Methyl-D-Aspartate Receptor Antagonists JENNIFER L. NARGI-AIZENMAN AND DIANE E. GRIFFIN, Johns Hopkins University, The Journal of Virology, 75(479), (2001)
Link To Paper

Abstract:
Virus infection of neurons leads to different outcomes ranging from latent and noncytolytic infection to celldeath. Viruses kill neurons directly by inducing either apoptosis or necrosis or indirectly as a result of the hostimmune response. Sindbis vi

Keywords:
Neuronal Death; apoptosis; necrosis; Sindbis virus

Materials & Methods:
Primary cortical cells were prepared from gestational day 18 Long-Evans rats as previously described (56). Briefly the cortex was dissected in Hanks balanced salt solution and digested in 10 U of papain (Worthington Biochemical Lakewood N.J.)/ml in

Microscopic Technique
Time Lapse Microscopy

Cell Type(s)
Primary