Technical Reference #478

478.

Light-Mediated Inhibition Of Protein Synthesis Michael Goard; Girish Aakalu; Olesya D. Fedoryak; Carlo Quinonez; Jamii St. Julien; Stephen J. Poteet; Erin M. Schuman;and Timothy M. Dore, University of Georgia, Chemistry & Biology, 12(478), (2005)
Link To Paper

Abstract:
Theregulation of protein synthesis is vital for a host of cell biological processes but investigating roles for protein synthesis have been hindered by the in-ability to selectively interfere with it. To inhibit protein synthesis with spatial and temp

Keywords:
Protein Synthesis; UV light; anisomycin;

Materials & Methods:
Dissociated hippocampal neuron cultures were prepared from postnatal 2- and 3-day rat pups as described [41]. Neurons were plated at a density of 15000–45000 cells/cm2 onto poly-L-lysine-coated cell-culture dishes (MatTek). The cultures were maintain

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
CHO