Technical Reference #477

477.

Na/Ca2 Exchanger Plays A Key Role In Inducing Apoptosis After Hypoxia In Cultured Guinea Pig Ventricular Myocytes B. N. Eigel; H. Gursahani; and R. W. Hadley, University of Kentucky, American Journal of Physiology: Heart and Circulatory Physiology, 287(477), (2004)
Link To Paper

Abstract:
Na /Ca2 exchanger plays a key role in inducing apoptosis after hypoxia incultured guinea pig ventricular myocytes. Am J Physiol Heart CircPhysiol 287: H1466–H1475 2004. First published May 20 2004;10.1152/ajpheart.00874.2003.—Altered Na /Ca2 ex

Keywords:
heart; endothelin; phenylephrine; ischemia

Materials & Methods:
Cultured adult myocytes were prepared using common sterile techniques (8 25). Isolated myocytes were suspended in serum-free medium 199 that was supplemented with (in mmol/l) 25 HEPES 5 creatine 2 L-carnitine 5 taurine and 104 insulin; also inclu

Microscopic Technique
Confocal Microscopy, Laser Scanning

Cell Type(s)
Myocytes