Technical Reference #473

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass-bottomed petri dishes (Mattek Corp.; Ashland; Mass.)

473.

Diminished Rev-Mediated Stimulation Of Human Immunodeficiency Virus Type 1 Protein Synthesis Is A Hallmark Of Human Astrocytes EVA LUDWIG; FRANCESCA CECCHERINI-SILBERSTEIN; JUTTA VAN EMPEL; VOLKER ERFLE; MARKUS NEUMANN; AND RUTH BRACK-WERNER, GSF-National Research Center for Environment and Health, The Journal of Virology, 73(473), (1999)
Link To Paper

Abstract:
Astrocytes are target cells for human immunodeficiency virus type 1 (HIV-1) in the central nervous system with attenuated virus replication in vivo and in vitro. In infected astrocytes viral gene expression is restrictedmainly to nonstructural (early)

Keywords:
Human Immunodeficiency Virus Type 1; Nef; TH4-7-5

Materials & Methods:
Microscopy of fixed and living cells expressing GFP fusion proteins was performed as described extensively in reference 23. For GFP analysis in living cells cells were seeded in glass-bottomed petri dishes (Mattek Corp. Ashland Mass.) transfected as

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
U87MG, U138MG;U373MG; HTB-14, -16, and -1; Hela; HEK 293