Technical Reference #472

472.

Nitric Oxide And Calcium Ions In Apoptotic Esophageal Carcinoma Cells Induced By Arsenite Zhong-Ying Shen; Wen-Ying Shen; Ming-Hua Chen; Jian Shen; Wei-Jie Cai; Zeng Yi, Shantou University, World Journal of Gastroenterology, 8(472), (2002)
Link To Paper

Abstract:
To Quantitatively analyze the nitri oxide (NO) andCa2+ in apoptosis of esophageal carcinoma cells inducedby arsenic trioxide (As2O3). The cell line SHEEC1 a malignant esophagealepithelial cell induced by HPV in synergy with TPA inour laboratory

Keywords:
Nitric oxide; calcium ions; arsenite; carcinogenic agent; apoptosis

Materials & Methods:
The cells were cultured on the coverslips within the glass bottom of a small cultured dish (No. 0 uncoated and irradiated. MatTek Co. USA). At the exponential growth period the cells were stained with 10µmol·L-1 fluo-3/AM (Molecular Probe) for 30mi

Microscopic Technique
Confocal Microscopy

Cell Type(s)
SHEEC1