Technical Reference #471

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
plastic Petri dish with a cover glass bottom (MatTek Corp., Ashland, MA, USA)

471.

Ageing-Associated Aberration In Meiosis Of Oocytes From Senescence-Accelerated Mice Lin Liu and David L.Keefe, Brown University, Human Reproduction, 17(10), 2679, (2002)
Link To Paper

Abstract:
The senescence-accelerated mouse (SAM) has been shown to exhibit ageing-associated mitochondrialdysfunction and oxidative stress, and early decline in fertility. METHODS: We compared meiotic progressionof germinal vesicle oocytes between young (2–3 mo

Keywords:
ageing, meiosis, oocyte, Pol-Scope, senescence-accelerated mouse

Materials & Methods:
Pol-Scope imaging for metaphase spindles were carried out as described previously (Liu et al., 2000). Oocytes were imaged using a Zeiss Axiovert 100 inverted microscope, equipped with a Cohu analogue video camera and Pol-Scope hardware consisting of li

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Oocytes