Technical Reference #470

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
MatTek glass-bottomed 35-mm dishes

470.

Agonist- And Protein Kinase C-Induced Phosphorylation Have Similar Functional Consequences For Gastrin-Releasing Peptide Receptor Signaling Via Gq ROXANNE A. ALLY; KIRK L. IVES; ELIE TRAUBE; IMAN ELTOUNSI; PEI-WEN CHEN; PATRICK J. CAHILL; JAMES F. BATTEY; MARK R. HELLMICH; and GLENN S. KROOG, Albert Einstein College of Medicine of Yeshiva University, Molecular Pharmacology, 64(470), (2003)
Link To Paper

Abstract:
Acute desensitization of many guanine nucleotide-binding protein-coupled receptors (GPCRs) requires receptor phosphorylationand subsequent binding of an arrestin. GPCRs are substratesfor phosphorylation by several classes of kinases.Gastrin-releas

Keywords:
guanine nucleotide-binding protein- coupled receptors; phosphorylation; carboxyl terminal domain; Acute desensitization; rhodopsin

Materials & Methods:
Stably transfected Balb 3T3 fibroblasts (expressing high levels of GRPr constructs) were plated in MatTek glass-bottomed 35-mm dishes at 150 to 200000 cells/dish. The next day each dish was transfected with 0.1 to 1 g of EGFP-N1- - arrestin1 (arr2)

Microscopic Technique
Confocal Microscopy

Cell Type(s)
3T3