Technical Reference #470

470.

Agonist- And Protein Kinase C-Induced Phosphorylation Have Similar Functional Consequences For Gastrin-Releasing Peptide Receptor Signaling Via Gq ROXANNE A. ALLY, KIRK L. IVES, ELIE TRAUBE, IMAN ELTOUNSI, PEI-WEN CHEN, PATRICK J. CAHILL, JAMES F. BATTEY, MARK R. HELLMICH, and GLENN S. KROOG, Albert Einstein College of Medicine of Yeshiva University, Molecular Pharmacology, 64(4), 893, (2003)
Link To Paper

Abstract:
Acute desensitization of many guanine nucleotide-binding protein-coupled receptors (GPCRs) requires receptor phosphorylationand subsequent binding of an arrestin. GPCRs are substratesfor phosphorylation by several classes of kinases.Gastrin-releas

Keywords:
guanine nucleotide-binding protein- coupled receptors, phosphorylation, carboxyl terminal domain, Acute desensitization, rhodopsin

Materials & Methods:
Stably transfected Balb 3T3 fibroblasts (expressing high levels of GRPr constructs) were plated in MatTek glass-bottomed 35-mm dishes at 150 to 200,000 cells/dish. The next day, each dish was transfected with 0.1 to 1 g of EGFP-N1- - arrestin1 (arr2)

Microscopic Technique
Confocal Microscopy

Cell Type(s)
3T3