Technical Reference #469

469.

Effect Of Propofol On Hypotonic Swelling– Induced Membrane Depolarization In Human Coronary Artery Smooth Muscle Cells Takako Masuda; M.D.; Yoshinobu Tomiyama; M.D.; Hiroshi Kitahata; M.D.; Yasuhiro Kuroda; M.D.; Shuzo Oshita; M.D., Tokushima University School of Medicine, Anesthesiology, 100(469), (2004)
Link To Paper

Abstract:
Stretch (mechanical stress)–induced membrane depolarization of smooth muscle may contribute to basal vasculartone and myogenic control. Propofol induces vasodilationand inhibits myogenic control. Hypotonic swelling was used asa model of mechanical

Keywords:
Hypotonic Swelling– induced Membrane Depolarization; basal vascular tone and myogenic control; propofol; volume-sensitive chloride channels; nonselective cation channels

Materials & Methods:
Human coronary artery smooth muscle cells which were certified to be a virus-free and a pure cell population on the basis of staining patterns for -actin were obtained from Cell Application Inc. (San Diego CA). The cells were routinely maintained

Microscopic Technique
Fluorescence Microscopy, Inverted Epifluorescence Microscopy

Cell Type(s)
SMOOTH