Technical Reference #468

468.

Reactive Oxygen Species Are Required For The Rapid Reactivation Of The Sodium-Calcium Exchanger In Hypoxic/Reoxygenated Guinea Pig Ventricular Myocytes B.N. Eigel; H. Gursahani; and R.W. Hadley, University of Kentucky, American Journal of Physiology: Heart and Circulatory Physiology, 286(468), (2004)
Link To Paper

Abstract:
The cardiac Na+-Ca2+ exchanger (NCX) contributes to cellular injury during hypoxia asits altered function is largely responsible for a rise in cytosolic [Ca2+]. In addition the NCX inguinea-pig ventricular myocytes undergoes profound inhibition duri

Keywords:
antioxidants; diazoxide; heart; hypoxia; ischemia

Materials & Methods:
Cultured adult myocytes were prepared using a sterile technique described previously (7). Isolated myocytes were suspended in serum-free Medium-199 which was supplemented with (mmol/L): 25 HEPES 5 creatine 2 L-carnitine 5 taurine and 10-4 insulin. 0

Microscopic Technique
Confocal Microscopy, Laser Scanning

Cell Type(s)
Myocytes