Technical Reference #467

467.

Acidification Of The Phagosome In Crassostrea Virginica Hemocytes Following Engulfment Of Zymosan AMY E. BEAVEN AND KENNEDY T. PAYNTER, University of Maryland, Biological Bulletin, 196, 27, (1999)
Link To Paper

Abstract:
Phagocytic hemocytes are responsible for engulfingand internally degrading foreign organisms withinthe hemolymph and tissue of the eastern oyster, Crassostreavirginica. Since rapid acidification of the phagosome lumenis typically essential for act

Keywords:
Crassostrea virginica, acidification, phagosome, oyster

Materials & Methods:
valve, draining the mantle fluid, and withdrawing hemolymph from the adductor muscle sinus using a lo-ml syringe equipped with a 22-gauge, 1.5-in. needle. Hemolymph from two or three oysters was expelled slowly into a glass test tube and kept on ice t

Microscopic Technique
Light Microscopy

Cell Type(s)
Hemocytes