Technical Reference #466

466.

Differential Effects Of Latrunculin-A On Myofibrils In Cultures Of Skeletal Muscle Cells: Insights Into Mechanisms Of Myofibrillogenesis Jushuo Wang; Jean M. Sanger; and Joseph W. Sanger, University of Pennsylvania, Cell Motility and the Cytoskeleton, 62(466), (2005)
Link To Paper

Abstract:
To test different models of myofibrillogenesis we followed live cells expressing Green Fluorescent Proteins ligated to either actin or alpha-actinin and analyzed stressfibers premyofibrils and myofibrils in quail myotube cultures. Actin filaments in

Keywords:
myofibrillogenesis; sarcomere; premyofibril; nascent myofibril; mature myofibril; actin; alpha-actinin; CapZ; latrunculin A

Materials & Methods:
Skeletal myoblasts were isolated from the breast muscles of 9-day-old quail embryos and plated on collagen- coated 35-mm MatTek (Ashland MA) dishes at concentrations of 105 cells per dish according to procedures described in Dabiri et al. [1999]. Fol

Microscopic Technique
Live Cell Microscopy

Cell Type(s)
Myoblasts