Technical Reference #465

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
poly-D-lysine-coated MatTek dishes. Specialized 35-mm culture dishes; each possessing a 13-mm hole to which a coverslip was fused (MatTek Corporation; Ashland; MA)

465.

Non-Coplanar 2;2;3;5;6-Pentachlorobiphenyl (Pcb 95) Amplifies Ionotropic Glutamate Receptor Signaling In Embryonic Cerebellar Granule Neurons By A Mechanism Involving Ryanodine Receptors Juliette Gafni; Patty W. Wong; and Isaac N. Pessah, University of California Davis, Toxicological Sciences, 77(465), (2004)
Link To Paper

Abstract:
The mechanisms by which non-coplanar 22 35 6-pentachlorobiphenyl(PCB 95) and rapamycin interact with ryanodine receptor(RyR) complexes to alter Ca2 signaling were explored inintact cerebellar granule neurons. PCB 95 (10 M 20 min) significant

Keywords:
polychlorinated biphenyls; cerebellar granule neurons; calcium signaling; glutamate excitotoxicity; ryanodine receptors

Materials & Methods:
Cultures enriched in cerebellar granule cells were prepared using a standard trypsin dispersion protocol (Trenkner 1991; Qiu et al. 1995). Cerebella from 7-day-old mice (C57BL/6; B&K Universal Fremont CA) were incubated in a Ca2-Mg2 -free PBS contai

Microscopic Technique
Fluorescence Microscopy, Inverted Epifluorescence Microscopy

Cell Type(s)
PC12