Technical Reference #464

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm dishes with a patterned glass coverslip (MatTek Corporation, MA)

464.

Induction Of Apoptosis In Cultured Endothelial Cells By A Cadherin Antagonist Peptide: Involvement Of Fibroblast Growth Factor Receptor-Mediated Signaling Noam Erez, Eli Zamir, Barbara J. Gour, Orest W. Blaschuk, and Benjamin Geiger, Weizmann Institute of Science, Experimental Cell Research, 294(464), 368, (2004)
Link To Paper

Abstract:
Cadherins are a family of transmembrane glycoproteins mediating calcium-dependent, homophilic cell–cell adhesion. In addition, thesemolecules are involved in signaling events, regulating such processes as cell motility, proliferation, and apoptosis. Mem

Keywords:
N-cadherin; Apoptosis; FGFR; Endothelial cells

Materials & Methods:
Cells were cultured until reaching confluence. After an additional 24 h, the medium was removed and the cells were incubated for 24–36 h with peptide-containing medium. Ten-micromolar BrdU was then added and the cells were permeabilized and fixed 45 m

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
HEK-293