Technical Reference #463

463.

Viewing Cell Movements In The Developing Neuroendocrine Brain STUART A. TOBET; HEATHER J. WALKER; MARIANNE L. SENEY; AND KWOK W. YU, Colorado State University, Integrative and Comparative Biology, 43(463), (2003)
Link To Paper

Abstract:
Many studies suggest that migratory guidance cues within the developing brain are diverseacross many regions. To better understand the early development and differentiation of select brain regionsan in vitro method was developed using selected inbred

Keywords:
brain development; GFP; ventromedial nucleus of the hypothalamus

Materials & Methods:
Heads or brains are dissected free from mouse embryos in cold Krebs buffer. After embedding in 8% low melting temperature agarose (Sigma Type VII-A) slices (250 mm thick) were cut using a vibrating microtome (Leica VT1000S) and placed into cold steri

Microscopic Technique
Video Microscopy

Cell Type(s)
Neurons