Technical Reference #462

462.

The Role Of A2E In Prevention Or Enhancement Of Light Damage In Human Retinal Pigment Epithelial Cells Joan E. Roberts; Barbara M. Kukielczak; Dan-Ning Hu; David S. Miller; Piotr Bilski; Robert H. Sik; Ann G. Motten and Colin F. Chignell, Fordham University, Photochemistry and Photobiology, 75(462), (2002)
Link To Paper

Abstract:
The process of sight (photostasis) produces as a by-producta chromophore called 2-[26-dimethyl-8-(266-trimethyl-1-cyclohexen-1-yl)-1E3E5E7E-octatetraenyl]-1-(2-hydroxyethyl)-4-[4-methyl-6-(266-trimethyl-1-cyclohexen-1-yl)-1E3E5E-hexatr

Keywords:
Light Damage; human retinal pigment epithelial (h-RPE) cells; A2E

Materials & Methods:
h-RPE cells were grown in 35 mm glassbottom microwell dishes (MatTek Ashland MA). To acquire images the dish containing the h-RPE cells was mounted on the stage of a Zeiss inverted confocal laser scanning microscope (Model 510 with multiphoton capa

Microscopic Technique
Confocal Microscopy

Cell Type(s)
h-RPE