Technical Reference #459

459.

The Presence Of Both The Amino- And Carboxyl-Terminal Domains In The Ar Is Essential For The Completion Of A Transcriptionally Active Form With Coactivators And Intranuclear Compartmentalization Common To The Steroid Hormone Receptors: A Three Dimensional MASAYUKI SAITOH; RYOICHI TAKAYANAGI; KIMINOBU GOTO; AKIYOSHI FUKAMIZU; ARIHIRO TOMURA; TOSHIHIKO YANASE; AND HAJIME NAWATA, Kyushu University, Molecular Endocrinology, 16(459), (2002)
Link To Paper

Abstract:
To clarify the physiological significance of the intranuclearspeckled distribution or foci formationof liganded steroid receptors the subnuclear distributionof green (GFP) yellow (YFP) and cyan(CFP) fluorescent protein-tagged receptors andc

Keywords:
Steroid Hormone Receptors; foci formation; GREEN FLUORESCENT protein

Materials & Methods:
The cells were cultured in 35-mm glass-bottom dishes (Mat- Tek) (3 105 cells/dish) and then transfected with various plasmids in a total amount of 0.5 g/dish using 2.5 l of SuperFect. For coexpression studies the total amount of vector added to e

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
COS-7