Technical Reference #458

458.

Stochasticity Of Manganese Superoxide Dismutase Mrna Expression In Breast Carcinoma Cells By Molecular Beacon Imaging Timothy J. Drake; Colin D. Medley; Arup Sen; Richard J. Rogers; and Weihong Tan, University of Florida, ChemBioChem, 6(458), (2005)
Link To Paper

Abstract:
Visual and quantitative monitoring of cell-to-cell variation in the expression of manganese superoxide dismutase (MnSOD) mRNAby using novel ratiometric imaging with molecular beacons (MB)reveals a distinct change in patterns following induction ofh

Keywords:
Breast Carcinoma; lipopolysaccharide; stochasticity; Molecular beacons

Materials & Methods:
MDA-MB-231 cells were maintained in Dulbecco’s Modification of Eagle’s Medium (DMEM Fisher Scientific Hampton NH) supplemented with fetal bovine serum (10%. Invitrogen Carlsbad CA) and Gentamycin (Sigma) in T75 Falcon flasks (Fisher Scientific) in

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
MDA-MB-231