Technical Reference #457

457.

Golgi Duplication In Trypanosoma Brucei Cynthia Y. He; Helen H. Ho; Joerg Malsam; Cecile Chalouni; Christopher M. West; Elisabetta Ullu; Derek Toomre; and Graham Warren, Ludwig Institute for Cancer Research, The Journal of Cell Biology, 165(457), (2004)
Link To Paper

Abstract:
Duplication of the single Golgi apparatus in the protozoan parasiteTrypanosoma bruceihas beenfollowed by tagging a putative Golgi enzyme and amatrix protein with variants of GFP. Video microscopyshows that the new Golgi appears de novo near to

Keywords:
Golgi apparatus; Duplication;Trypanosoma brucei; de novo biogenesis

Materials & Methods:
T. brucei cells were plated on the surface of agarose gels prepared in cultivation medium and air dried briefly to limit cell motility. The gel was placed in an imaging dish (MatTek) with the cell side facing the coverslip. Cells were imaged at 28 C in

Microscopic Technique
Video Microscopy, Immunofluorescence

Cell Type(s)
T. brucei