Technical Reference #455

455.

Epidermal Growth Factor Protects Rat Epithelial Cells Against Acid-Induced Damage Through The Activation Of Na1/H1 Exchangers OSAMU FURUKAWA; HIROSHI MATSUI; NORIKO SUZUKI and SUSUMU OKABE, Kyoto Pharmaceutical University, The Journal of Pharmacology and Experimental Therapeutics, 288(455), (1999)
Link To Paper

Abstract:
We examined the effect of epidermal growth factor (EGF) on acid-induced cell damage in rat gastric epithelial cells (RGM1)and investigated the mechanisms of this effect. Cells wereincubated with EGF for 5 15 30 and 60 min and then immersedin an

Keywords:
epidermal growth factor; cell damage; acid

Materials & Methods:
Changes in the pHi of RGM1 cells were detected with an image analyzer (Attofluor Ratio- Vision Karl Zeiss Co. Ltd. Obernkochen Germany). RGM1 cells cultured in a glass-bottomed culture dish (MatTek Co. Ashland MA) were incubated with 0.3 mM BCEC

Cell Type(s)
RGM1