Technical Reference #454

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass bottom in a polyl-lysine coated petri dish (MatTek; Ashland, MA)

454.

Fluorogenic Substrate [Ala-Pro] 2-Cresyl Violet But Not Ala-Pro-Rhodamine 110 Is Cleaved Specifically By Dppiv Activity: A Study In Living Jurkat Cells And Cd26/Dppiv-Transfected Jurkat Cells Emil Boonacker, Sjoerd Elferink, Abdennasser Bardai, Bernard Fleischer, and Cornelis J.F. Van Noorden, University of Amsterdam, The Journal of Histochemistry & Cytochemistry, 51(454), 962, (2003)
Link To Paper

Abstract:
Fluorogenic substrates [Ala-Pro]2-cresyl violet and Ala-Pro-rhodamine 110have been tested for microscopic detection of protease activity of dipeptidyl peptidase IV(DPPIV) in living cells. DPPIV activity is one of the many functions of the multifun

Keywords:
living cell cytochemistry, protease, metabolic mapping, biocomplexity, CD26/DPPIV, Jurkat cells, confocal scanning laser microscopy, fluorescence microscopy

Materials & Methods:
Confocal analysis was performed to localize DPPIV-like activity in living Jurkat and transfected Jurkat cells on a Leica SP 2 AOBS confocal microscope (Leica Microsystems; Mannheim, Germany). In case of the use of [Ala-Pro] 2 -cresyl violet, fluor

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Jurkat