Technical Reference #453

453.

Using Green Fluorescent Malaria Parasites To Screen For Permissive Vector Mosquitoes Friedrich Frischknecht; Beatrice Martin; Isabelle Thiery; Catherine Bourgouin and Robert Menard, Institut Pasteur, Malarial Journal, 5(453), (2006)
Link To Paper

Abstract:
The Plasmodium species that infect rodents particularly Plasmodium berghei andPlasmodium yoelii are useful to investigate host-parasite interactions. The mosquito species that actas vectors of human plasmodia in South East Asia Africa and South Ame

Keywords:
malaria; sporozoites; Plasmodium berghei

Materials & Methods:
Whole mosquitoes or isolated midguts were observed at 10–18 days (A. stephensi and A. gambiae) or 10–26 days (A. albimanus) after the infectious blood meal. Sporozoites were isolated from infected salivary glands 15–22 days (A. stephensi) and 15–35 da

Microscopic Technique
Fluorescence Microscopy, Time Lapse

Cell Type(s)
MOSQUITOES