Technical Reference #452

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
sterile glass-bottom 35-mm dishes coated with poly(D-lysine) (MatTek; Ashland; MA)

452.

Recycling Cd1D1 Molecules Present Endogenous Antigens Processed In An Endocytic Compartment To Nkt Cells Tonya J. Roberts; Venkataraman Sriram; Philip M. Spence; Ming Gui; Kyoko Hayakawa; Igor Bacik; Jack R. Bennink; Jonathan W. Yewdell; and Randy R. Brutkiewicz, Indiana University, The Journal of Immunology, 168(452), (2002)
Link To Paper

Abstract:
Mouse CD1d1 molecules present endogenous glycolipids to NKT cells. Although glycolipid presentation requires CD1d1 transportthrough the endocytic pathway the processing requirements for such endogenous Ag presentation by CD1d1 molecules areundefined.

Keywords:
Endogenous Antigens; CD1d1 molecules; pH; glycolipid

Materials & Methods:
P13.9 cells were plated in sterile glass-bottom 35-mm dishes coated with poly(D-lysine) (MatTek Ashland MA) at a density of 1 107 cells/dish. After overnight adherence the cells were infected with VV-CD1d1WT VV-CD1d1TD or VV-CD1d1Y322A at an MOI

Microscopic Technique
Confocal Microscopy

Cell Type(s)
P13.9