Technical Reference #449

449.

Regulation Of Egf Receptor Signaling By The Marvel Domain-Containing Protein Cklfsf8 Caining Jin; Peiguo Ding; Ying Wang; Dalong Ma, Peking University, FEBS Letters, 579(449), (2005)
Link To Paper

Abstract:
It is known that chemokine-like factor superfamily 8(CKLFSF8) a member of the CKLF superfamily has four putativetransmembrane regions and a MARVEL domain. Its structureis similar to TM4SF11 (plasmolipin) and widely distributedin normal tissue. H

Keywords:
CKLFSF8; EGFR; Signal internalization

Materials & Methods:
· 103 cells/well were seeded into MatTek Cultureware (MatTek Corporation USA). After a 4 h incubation at 37 C 1 ml fresh medium was supplemented. At 24 h post-transfection cells were serum-deprived for 20 h and stimulated with 10 ng/ml EGF for the

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
HEK-293