Technical Reference #448

448.

Subtype-Specific Differences In Subcellular Localization Of A1-Adrenoceptors: Chlorethylclonidine Preferentially Alkylates The Accessible Cell Surface A1-Adrenoceptors Irrespective Of The Subtype AKIRA HIRASAWA; TATSUO SUGAWARA; TAKEO AWAJI; KEIKO TSUMAYA; HIROSHI ITO; and GOZOH TSUJIMOTO, National Children's Medical Research Center, Molecular Pharmacology, 52(448), (1997)
Link To Paper

Abstract:
Selective inactivation of a1B-adrenoceptor (AR) by the sitedirectedalkylating agent chlorethylclonidine (CEC) has beenused as one of major pharmacological criteria to subclassifya1-AR; however the mechanism for the differential CEC sensitivityof

Keywords:
a1B-adrenoceptor (AR); anti-amino terminus antibodies

Materials & Methods:
Transfected and untransfected COS-7 cells were seeded at 1 3 105 per well of the cover-glass bottom culture dish (MatTek Ashland MA) in 2.0 ml of medium. Two days after transfection cells were washed with PBS and examined using LSMGB200 within 30 m

Microscopic Technique
Confocal Microscopy

Cell Type(s)
COS-7