Technical Reference #448

448.

Subtype-Specific Differences In Subcellular Localization Of A1-Adrenoceptors: Chlorethylclonidine Preferentially Alkylates The Accessible Cell Surface A1-Adrenoceptors Irrespective Of The Subtype AKIRA HIRASAWA, TATSUO SUGAWARA, TAKEO AWAJI, KEIKO TSUMAYA, HIROSHI ITO, and GOZOH TSUJIMOTO, National Children's Medical Research Center, Molecular Pharmacology, 52(448), 766, (1997)
Link To Paper

Abstract:
Selective inactivation of a1B-adrenoceptor (AR) by the sitedirectedalkylating agent chlorethylclonidine (CEC) has beenused as one of major pharmacological criteria to subclassifya1-AR; however, the mechanism for the differential CEC sensitivityof

Keywords:
a1B-adrenoceptor (AR), anti-amino terminus antibodies

Materials & Methods:
Transfected and untransfected COS-7 cells were seeded at 1 3 105 per well of the cover-glass bottom culture dish (MatTek, Ashland, MA) in 2.0 ml of medium. Two days after transfection, cells were washed with PBS and examined using LSMGB200 within 30 m

Microscopic Technique
Confocal Microscopy

Cell Type(s)
COS-7